杨 辉

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杨 辉

2023-11-03 21:07| 来源: 网络整理| 查看: 265

Gao, N., Hu. J., He, B., Ji, Z., Hu, X., Huang, J., Wei, Y., Peng, J., Wei, Y., Zhou, Y., Shen, X., Li, H., Feng, X., Xiao, Q., Shi, L., Sun, Y., Zhou, C., Zhou, H.* & Yang, H.* (2021) Endogenous promoter-driven sgRNA for monitoring the expression of low-abundant genes and lncRNAs. Nat. Cell Biol. In press.

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Zuo, E., Sun, Y., Wei, W., Yuan, T., Ying, W., Sun, H., Yuan, L., Steinmetz, L.*, Li, Y.* & Yang, H.* (2020) GOTI, a method to identify genome-wide off-target effects of genome editing in mouse embryos. Nat. Protoc. 15: 3009–3029.

Zuo, E.*, Sun, Y., Yuan, T., He, B., Zhou, C., Ying, W., Liu, J., Wei, W., Zeng, R., Li, Y.* & Yang, H.* (2020) A rationally-engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects. Nat. Methods 17: 600–604.

Lin, X., Chen, H., Lu, Y., Hong, S., Hu, X., Gao, Y., Lai, L., Li, J., Wang, Z., Ying, W., Ma, L., Wang, N., Zuo, E.*, Yang, H.* & Chen, W.* (2020) Base editing-mediated splicing correction therapy for spinal muscular atrophy. Cell Res. 30: 548–550.

He, B., Peng, W., Huang, J., Zhang, H., Zhou, Y., Yang, X., Liu, J., Li, Z., Xu, C., Xue, M., Yang, H.* & Huang, P.* (2020) Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver. Protein Cell 11: 518–524.

Zhou, C., Hu, X., Tang, C., Liu, W., Wang, S., Zhou, Y., Zhao, Q., Bo, Q., Shi, L., Sun, X.*, Zhou, H.* & Yang, H.* (2020) CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration. Natl. Sci. Rev. 7: 835–837.

Zhou, H.*, Su, J., Hu, X., Zhou, C., Li, H., Chen, Z., Xiao, Q., Wang, B., Wu, W., Sun, Y., Zhou, Y., Tang, C., Liu, F., Wang, L., Feng, C., Liu, M., Li, S., Zhang, Y., Xu, H., Yao, H., Shi, L. & Yang, H.* (2020) Glia-to-neuron conversion by CRISPR-CasRx alleviates symptoms of neurological disease in mice. Cell 181: 590–603.

Li, J., Lin, X., Tang, C., Lu, Y., Hu, X., Zuo, E., Li, H., Ying, W., Sun, Y., Lai, L., Chen, H., Guo, X., Zhang, Q., Wu, S., Zhou, C., Shen, X., Wang, Q., Lin, M., Ma, L., Wang, N., Krainer A., Shi, L.*, Yang, H.* & Chen, W.* (2020) Disruption of splicing-regulatory elements using CRISPR/Cas9 rescues spinal muscular atrophy in human iPSCs and mice. Natl. Sci. Rev. 7: 92–101.

Yang, G., Zhou, C., Wang, R., Huang, S., Wei, Y., Yang, X., Liu, Y., Li, J., Lu, Z., Ying, W., Li, X., Jing, N., Huang, X.*, Yang, H.* & Qiao, Y.* (2019) Base-editing-mediated R17H substitution in histone H3 reveals methylation-dependent regulation of Yap signaling and early mouse embryo development. Cell Rep. 26: 302–312.

Zuo, E., Sun, Y., Wei, W., Yuan, T., Ying, W., Sun, H., Yuan, L., Steinmetz, L.*, Li, Y.* & Yang, H.* (2019) Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos. Science 364: 289–292.

Wang, H.* & Yang, H.* (2019) Gene-edited babies: What went wrong and what could go wrong. PLOS Biol. 17: e3000224. (Perspective)

Zhang, M., Zhou, C., Wei, Y., Xu, C., Pan, H., Ying, W., Sun, Y., Sun, Y., Xiao, Q., Yao, N., Zhong, W., Li, Y., Wu, K., Yuan, G., Mitalipov, S.*, Chen, Z.* & Yang, H.* (2019) Human cleaving embryos enable robust homozygotic nucleotide substitutions by base editors. Genome Biol. 20: 101.

Zhou, C., Sun, Y., Yan, R., Liu, Y., Zuo, E., Gu, C., Han, L., Wei, Y., Hu, X., Zeng, R., Li, Y.*, Zhou, H.*, Guo, F.* & Yang, H.* (2019) Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis. Nature 571: 275–278.

Liu, Y., Li, J., Zhou, C., Meng, B., Wei, Y., Yang, G., Lu, Z., Shen, Q., Zhang, Y., Yang, H.* & Qiao, Y.* (2019) Allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci using Staphylococcus aureus Cas9 (SaCas9). Sci. Bull. 64: 1592–1600.

Yang, H.* (2019) Funding research, a Chinese perspective. Genome Biol. 20: 177. (Editorial)

Hu, X., Wang, J., Yao, X., Xiao, Q., Xue, Y., Wang, S., Shi, L., Shu, Y.*, Li, H.* & Yang, H.* (2019) Screened AAV variants permit efficient transduction access to supporting cells and hair cells. Cell Discov. 5: 49.

Li, J., Hong, S., Chen, W.*, Zuo, E.* & Yang, H.* (2019) Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing. J. Genet. Genomics 46: 513–521. (Review)

Yao, X., Liu, Z., Wang, X., Wang, Y., Nie, Y., Lai, L., Sun, R., Shi, L., Sun, Q.* & Yang, H.* (2018) Generation of knock-in cynomolgus monkey via CRISPR/Cas9 editing. Cell Res. 28: 379–382.

Yao, X., Wang, X., Liu, J., Shi, L., Huang, P.* & Yang, H.* (2018) CRISPR/Cas9-mediated targeted integration In Vivo using a homology-mediated end joining-based strategy. J. Vis. Exp. 133: e56844.

Zhou, H., Liu, J., Zhou, C., Gao, N., Rao, Z., Li, H., Hu, X., Li, C., Yao, X., Shen, X., Sun, Y., Wei, Y., Liu, F., Ying, W., Zhang, J., Tang, C., Zhang, X., Xu, H., Shi, L., Cheng, L., Huang, P.* & Yang, H.* (2018) In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator transgenic mice. Nat. Neurosci. 21: 440–446.

Yao, X.*, Zhang, M., Wang, X., Ying, W., Hu, X., Dai, P., Meng, F., Shi, L., Sun, Y., Yao, N., Zhong, W., Li, Y., Wu, K., Li, W.*, Chen, Z.* & Yang, H.* (2018) Tild-CRISPR allows for efficient and precise gene knockin in mouse and human cells. Dev. Cell 45: 526–536.

Zhang, H., Pan, H., Zhou, C., Wei, Y., Ying, W., Li, S., Wang, G., Li, C., Ren, Y., Li, G., Ding, X., Sun, Y., Li, G., Song, L., Li, Y., Yang, H.* & Liu, Z.* (2018) Simultaneous zygotic inactivation of multiple genes in mouse through CRISPR/Cas9-mediated base editing. Development 145: dev168906.

Yao, X., Wang, X., Liu, J., Hu, X., Shi, L., Shen, X., Ying, W., Sun, X., Wang, X., Huang, P.* & Yang, H.* (2017) CRISPR/Cas9–mediated precise targeted integration In Vivo using a double cut donor with short homology arms. EBioMedicine 20: 19–26.

Yao, X., Wang, X., Hu, X., Liu, Z., Liu, J., Zhou, H., Shen, X., Wei, Y., Huang, Z., Ying, W., Wang, Y., Nie, Y., Zhang, C., Li, S., Cheng, L., Wang, Q., Wu, Y., Huang, P., Sun, Q.*, Shi, L.* & Yang, H.* (2017) Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 27: 801–814.

Zuo, E., Cai, Y., Li, K., Wei, Y., Wang, B., Sun, Y., Liu, Z., Liu, J., Hu, X., Wei, W., Huo, X., Shi, L., Tang, C., Liang, D., Wang, Y., Nie, Y., Zhang, C., Yao, X., Wang, X., Zhou, C., Ying, W., Wang, Q., Chen, R., Shen, Q., Xu, G., Li, J., Sun, Q.*, Xiong, Z.* & Yang, H.* (2017) One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs. Cell Res. 27: 933–945.

Zhou, C., Zhang, M., Wei, Y., Sun, Y., Sun, Y., Pan, H., Yao, N., Zhong, W., Li, Y., Li, W.*, Yang, H.* & Chen, Z.* (2017) Highly efficient base editing in human tripronuclear zygotes. Protein Cell 8: 772–775.

Zuo, E., Huo, X., Yao, X., Hu, X., Sun, Y., Yin, J., He, B., Wang, X., Shi, L., Ping, J., Wei, Y., Ying, W., Wei, W., Liu, W., Tang, C., Li, Y., Hu, J.* & Yang, H.* (2017) CRISPR/Cas9-mediated targeted chromosome elimination. Genome Biol. 18: 224.

Miao, L., Yao, H., Li, C., Pu, M., Yao, X., Yang, H., Qi, X., Ren, J.* & Wang, Y.* (2016) A dual inhibition: microRNA-552 suppresses both transcription and translation of cytochrome P450 2E1. Bba-Gene Regul. Mech. 1859: 650–662.

Yang, H., Wang, H. & Jaenisch,, R.* (2014) Generating genetically modified mice using CRISPR/Cas-mediated genome engineering. Nat. Protoc. 9: 1956–1968.

Yang, H., Wang, H., Shivalila, C., Cheng, A., Shi, L. & Jaenisch, R.* (2013) One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell 154: 1370–1379.

Cheng, A., Wang, H., Yang, H., Shi, L., Katz, Y., Theunissen, T., Rangarajan, S., Shivalila, C., Dadon, D. & Jaenisch, R.* (2013) Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 23: 1163–1171.

Yang, H., Liu, Z., Ma, Y., Zhong, C., Yin, Q., Zhou, C., Shi, L., Cai, Y., Zhao, H., Wang, H., Tang, F., Wang, Y., Zhang, C., Liu, X., Lai, D., Jin, Y.*, Sun, Q.* & Li, J.* (2013) Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes. Cell Res. 23: 1187–1200.

Jiang, J., Lv, W., Ye, X., Wang, L., Zhang, M., Yang, H., Okuka, M., Zhou, C., Zhang, X., Liu, L.* & Li, J.* (2013) Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation. Cell Res. 23: 92–106.

Wang, H., Yang, H., Shivalila, C., Dawlaty, M., Cheng, A., Zhang, F. & Jaenisch, R.* (2013) One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell 153: 910–918.

 Shi, L., Yang, H. & Li, J.* (2012) Haploid embryonic stem cells: an ideal tool for mammalian genetic analyses. Protein Cell 3: 806–810. (Review)

Yang, H., Shi, L., Wang, B., Liang, D., Zhong, C., Liu, W., Nie, Y., Liu, J., Zhao, J., Gao, X., Li, D., Xu, G.* & Li, J.* (2012) Generation of Genetically Modified Mice by Oocyte Injection of Androgenetic Haploid Embryonic Stem Cells. Cell 149: 605–617.

Lin, J., Shi, L., Zhang, M., Yang, H., Qin, Y., Zhang, J., Gong, D., Zhang, X., Li, D. & Li, J.* (2011) Defects in Trophoblast Cell Lineage Account for the Impaired In Vivo Development of Cloned Embryos Generated by Somatic Nuclear Transfer. Cell Stem Cell 8: 371–375.

Yang, H., Shi, L., Chen, C. & Li, J.* (2011) Mice generated after round spermatid injection into haploid two-cell blastomeres. Cell Res. 21: 854–858.

Jiang, J., Ding, G., Lin, J., Zhang, M., Shi, L., Lv, W., Yang, H., Xiao, H., Pei, G., Li, Y., Wu, J.* & Li, J.* (2011) Different developmental potential of pluripotent stem cells generated by different reprogramming strategies. J. Mol. Cell Biol. 3: 197–199.

Gu, T., Guo, F., Yang, H., Wu, H., Xu, G., Liu, W., Xie, Z., Shi, L., He, X., Jin, S., Iqbal, K., Shi, Y., Deng, Z., Szabó, P., Pfeifer, G., Li, J.* & Xu, G.* (2011) The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes. Nature 477: 606–610.

Yang, H., Shi, L., Zhang, S., Lin, J., Jiang, J. & Li, J. * (2010) High-efficiency somatic reprogramming induced by intact MII oocytes. Cell Res. 20: 1034-1042



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